The SARS‐CoV‐2 Spike Glycoprotein Directly Binds Exogeneous Sialic Acids: A NMR View

The interaction of the SARS CoV2 spike glycoprotein with two sialic acid-containing trisaccharides (α2,3 and α2,6 sialyl N-acetyllactosamine) has been demonstrated by NMR. NMR-based distinction between signals those acids in glycans covalently attached to protein belonging exogenous α2,3 N-acetyllactosamine ligands achieved synthesizing uniformly 13C-labelled at acid galactose moieties. STD-1H,13C-HSQC NMR experiments elegantly demonstrate direct residues both additional participation moieties, especially for α2,3-linked analogue. Additional presence a specific antibody N-terminal domain isolated receptor binding domains unambiguously show that site is located domain.